In vitro fertilization is the development of eggs, the collection of eggs, the integration of eggs into the laboratory sperm, and the placement of embryos in the uterus.
Development of ovules
Excess egg development is achieved by using various medicines on the ovaries. Normally in females there are about 15 eggs in a cycle, but only one develops. In the case of in vitro fertilization, the higher the number, the higher the chance of success of tube bebel treatment. In addition to medicines, needles are used in IVF treatment. On the second or third day of Reglin, these drugs and needles are used for 8-14 days. Additional medication is also administered to provide brain pressure on the ovaries either before or in conjunction with the treatment.
The eggs are developed in follicles. It is not seen whether or not eggs are present in these follicles. Sometimes it is follicle, but it may not have developed in egg. Follicles secrete estradiol hormone in direct proportion to their growth and development. In certain days of treatment, this hormone is followed and the development of the ovaries is monitored. The time for collecting the eggs is determined by the maturation (cracking) needle. Eggs are collected 34-36 hours after injecting the needle.
Collection of Eggs
The patient should not consume food and water after 24:00 pm before the procedure comes. In addition, perfume, deodorant and makeup material without the use of egg collection process should be reached. Sexual abstinence is required for sperm retrieval 3-5 days before egg collection. In the morning of collecting eggs, come to the center with the wife. While under general anesthesia, the egg is collected from the vaginal route, the spouse gives sperm in another room. Egg collection with the help of ultrasonography is inserted into the follicles with a single needle. Their contents are aspirated in a tube containing special solution and delivered to the embryo waiting to be ready. Collected eggs are placed in special dolaba called incubator. After picking up the eggs, the patient may be a bit drowsy or have a spicum. It usually heals within 2 hours and it will be able to go home. In the pubic area 2-3 days after the procedure, there may be gray pain and sometimes slight bleeding as regl pain.
Combining Sperm in Laboratory with Ovaries (Fertilization)
The procedure followed by in vitro fertilization or microinjection may differ.
Classic IVF (Sperm Infant Method): It is expected that sperm will be left in a certain amount next to each egg that is collected and it will enter the sperm egg itself and perform fertilization. The fertilized egg cells divide and Hcg also improves the embryo.2-3 In laboratory conditions embryos are regularly controlled and growth is ensured. This method is preferred by those with congestion in their tubes. However, since the fertility rate is low, microinjection method is preferred.
Assisted Hatching: The procedure performed by the embryologist, the dermis located at the outermost of the eggs is opened by the laser under the microscope, making it easier for the embryo to come out before placing it in the uterine wall.
Placement of Embryos in the Maternal Fetus
2 or 3 of the embryos that are formed 2-3 days or 5 days after the ovum collection and fertilization take place are placed in the operating theater with a special easy catheter like a normal gynecologic examination. After about 10 minutes, the patient is rested for about 1 hour. After that, he has to go home and rest. sexual intercourse is prohibited in this process until the pregnancy test. In order to maintain the pregnancy, the patient is given a suppository, hCG injections or needle containing progesterone hormone. On the day of testing, the placenta is made up of beta- HCG. If the value is within the specified range, the test is repeated 2 days later. After 2 weeks, USG is used to look for pregnancy cases or cuts in the uterus.
More than 3 healthy embryos can be obtained in egg collection. These embryos can be stored for up to 5 years and then reused. Frosted embryos at -196 ° C are stored on special conditions and are placed back into the patient’s womb when requested. Thus, transactions will not have to be done from the beginning. In this case, however, the chances of success decline by 10%. Because the embryos can not go out after being solved.